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plsX [2019-06-12 15:09:30]
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plsX [2019-06-12 15:09:30]

acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)
locus
BSU15890
pI
5.49
mw
35.61 kDa
protein length
333 aa Sequence Blast
gene length
1002 bp Sequence Blast
function
biosynthesis of phospholipids
product
acyl-acyl carrier protein (ACP):phosphate acyltransferase
essential
yes
ec
2.3.1.n2
synonyms
ylpD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,662,547 1,663,548

    Phenotypes of a mutant

  • essential [Pubmed|24224907,12682299]
  • depletion results in sporulation deficiency (via [protein|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|SpoIIR]) [Pubmed|22111992]
  • a ts mutant forms an abberant Z-ring and has a [SW|cell division] defect [Pubmed|24224907]

    • The protein

    Catalyzed reaction/ biological activity

  • catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)

    • Protein family

  • plsX family (according to Swiss-Prot)

    • Structure

  • [PDB|1VI1] [Pubmed|16021622]

    • [SW|Localization]

  • uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well [Pubmed|26816052]
  • in the presence of daptomycin, PlsX delocalizes from the membrane to the cytoplasm [Pubmed|27791134]

    • Expression and Regulation

    Operons

    genes
    [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]-[gene|8F5612395EBF90BEF4C80056EECAFC572384E7F8|plsX]-[gene|BB8542A6444564B21FF5DB6E2C75192C6DA2DFB8|fabD]-[gene|439B468A13137000FB42E9389391CB4986FFED84|fabG]-[gene|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA]
    description
    [Pubmed|19850612]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16932747], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]: repression, [Pubmed|12737802], in [regulon|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]) [Pubmed|16091051], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]

    • regulation

  • ''[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]'': repressed in the absence of malonyl-CoA or malonyl-[protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|ACP] ([protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]) [Pubmed|12737802]
  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]
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    Biological materials

    two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in Matsumoto lab [Pubmed|19282621]

    • References

  • 12737802,19282621,17645809,17557823,8759840,16932747,19850612,16091051,21383089,22111992,24224907,26816052,16021622,27791134